geneye UPHO User Manual - User Manual

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UPHO

ULTIMATE HOMOGENIZER

USER MANUAL

Table of content

1. Precautions

2. Safety Information

- Proper Use

- Electrical Safety

- Environment

- Biological Safety

- Personal Protective Equipment (PPE)

3. Introduction

4. Main Technical Parameters

- Structure Overview

5. Installation Procedures

- Unpacking and Packing List

- Removal of the Transport Security System

- Sample Adapter Installation

6. Operating Procedures

Automatic Program

- User-defined Program

7. Cleaning & User Maintenance

8. Troubleshooting

9. Warranty Statement

10. Disposal

- End of Life Disposal

11. Relevant Standards & Regulatory Requirements

- EC Declaration of Conformity

- Regulations

12. UPHO Accessories

1. Precautions

Please read, understand and practice the following precautions when

operating instructions your UPHO.

Know the limitations and hazards associated with the use of this

equipment.

• Do not operate this equipment in an environment where other

unshielded devices are being used, that radiate electromagnetic radiation.

• Do not use sharp objects such as a pencil point or ballpoint pen to

intreact with the touch screen, as damage may result. Please use a suitable

soft point stylus or your finger tip.

• This equipment should only be operated in temperatures between 10º C

to 40º C, transported and stored in temperatures between 0º C to 50º C,

with relative humidity less than 70%.

• Do not disassemble, modify or remodel the unit or accessories.

• This device should be kept out of the reach of children.

2. Safety Information

Before using UPHO, it is essential you read this user manual carefully and

pay particular attention to the safety information.

The instructions and safety information in the user manual must be

followed at all times to ensure a safe operation and to maintain UPHO in a

safe operating condition.

The advice given in this manual is intended to supplement, not supersede,

the normal safety requirements prevailing in the user’s country.

Proper Use

This product is intended to be used for research purposes only.

Other applications are not covered by Geneye warranty. The product is not

suitable for use in diagnostic or therapeutic applications.

Improper use of UPHO may cause personal injuries or damage to the

instrument. Geneye shall not be held responsible for any injuries or

damages resulting from non-observance of the operating instructions and

safety information in this manual.

If any abnormality, such as excessive noise, excessive vibration or

over-heating is observe during operation, please immediately shut off the

power at the main switch (rear of instrument) or at the electrical power

socket and call for professional service.

• Do not attempt to move UPHO during operation.

• UPHO should only be operated by suitably qualified and trained

personnel in accordance with Good Laboratory Practice.

• Do not make any modifications of the instrument.

• Disassembly will void the warranty.

• UPHO should only be used in combination with Geneye accessories, such

as sample adapters and spare parts.

• Do not continue to use UPHO or UPHO accessory if damage is occurred.

• Damage or personal injury can occur if UPHO sample adapter set is not

securely installed before operation (see User Manual Section 5.3).

• The use of sample adapters other than the Geneye sample adapters

described in this user manual may lead to the UPHO being damaged.

• Avoid spilling water or chemicals onto or into UPHO. Damage caused by

water or chemical spillage will void the warranty.

• Do not leave UPHO unattended during operation.

• The machine must be placed on a flat laboratory bench (preferably solid

wood or stone countertops) to minimise vibration during operation.

• The machine is designed for frequent use with short run times (typical

run time is 1 minute). Do not use long run times (>5 minutes) when

operating UPHO.

• The UPHO accessories (e.g. adapters, cans, beads) may become worn,

depending on the frequency of use and the material to be homogenized.

They should be regularly checked for wear and tear and replaced if

necessary.

Electrical Safety

To ensure satisfactory and safe operation of your UPHO, please follow the

safety instructions below at all times:

• Do not attempt to adjust or replace any internal parts of your UPHO - if

there is a fault, please call for professional service.

• When UPHO is not in use, switch it off at the power switch, on the back

panel of the unit (please see picture on page 7).

• If UPHO will not be out-of-use for a long period of time, switch off the

power switch, on the back panel of the unit and unplug the power cord

from the power outlet.

• To protect the internal circuits and parts from damage, please do not use

running water to clean your UPHO: use a soft damp cloth.

Environment

• Excessive exposure to direct sunlight may bleach exposed parts of the

instrument and eventually cause damage to plastic parts. UPHO should be

located out of direct sunlight.

Biological Safety

• Samples may contain infectious agents. You should be aware of the

health hazard presented by such agents and should use, store, and dispose

of such samples according to the required safety regulations.

• The laboratory manager must take the necessary precautions to ensure

that the surrounding workplace is safe, and that UPHO operators are

suitably trained and not exposed to hazardous levels of infectious agents.

• When working with volatile solvents, toxic substances, etc., you must

provide an efficient laboratory ventilation system to remove steams that

may be produced.

Personal Protective Equipment (PPE)

• Operators must wear the appropriate level of personal protective

equipment as required for the particular laboratory environment in which

the System will be used. This may include: laboratory coat, gloves, safety

glasses and safety shoes.

• Operators must not operate the equipment if feeling unwell.

3. Introduction

The traditional sample grinding with a Pestle & Mortar is a very slow

method, that can only process one single sample at a time and has a high

risk of causing cross-contamination. Besides that, after such a procedure, it

is necessary to clean and disinfect the equipment. Efficient sample

disruption/grinding is a prerequisite for nucleic acid, protein and other

biomolecules extraction and purification procedures.

Incomplete sample disruption can lead to significantly reduced recovery

yields of the molecules of interest and can increase the risk of clogging

when using purification columns or magnetic particles later in the

workflow.

UPHO is a versatile bead mill device designed for rapid, efficient and

simultaneous disruption of up to 64 samples. A wide range of sample

adapters are available for UPHO providing sample flexibility and leading to

reliable and quality results.

This bench device is one of the most advanced sample preparation

systems available today. UPHO was specifically developed for routine as

well as difficult and resistant samples, compatible with 3 types of grinding

and lyses thoroughly and quickly a wide variety of biological samples

including plant and animal tissues, cells, bacteria and yeast.

Each sample is simultaneously disrupted and homogenized by high-speed

shaking with a bead/beads in a sealed tube (bead milling), allowing an easy

and reproducible isolation of stable RNA, active proteins and full-length

genomic DNA.

The ergonomic design of the instrument ensures an easy loading of the

disposable centrifuge sample tubes that remain securely sealed during the

grinding process, effectively avoiding sample cross-contamination.

The homogenization speed (frequency) and duration run-times are

digitally controlled via UPHO’s intuitive touch screen, improving the

consistency for multi sample lots.

The different beads sizes (0,1 – 15 mm) and bead materials (wolfram steel,

metal, glass and ceramic) makes the grinding process more complete and

efficient with UPHO when compared to other available methods.

Moreover, thanks to UPHO’s design and options for the use of cryogenic

liquids to freeze samples, the sample temperature remains constant

throughout the homogenization process.

4. Main Technical Parameters

• Run-time setting: 1 (sec) - 9999 (min)

• Oscillation frequency setting: 1 – 70 Hz

• Acceleration: <2 seconds to reach maximum speed

• Power rating: 195 W

• Power requirement: 220 V - 240 V~, 50 Hz, 2.5 A, 375 W

• UPHO dimensions: � 460 × 460 × 530 mm (length × width × height)

• Weight: 35 kg

• Operating temperature: 10

ºC

~ 40

ºC

(relative humidity: �70%)

• Storage temperature: -20

ºC

~ 50

ºC

(relative humidity: �93%)

• Maximum noise emission: 65 dB

• Interface: Touch screen

Structure Overview - Front

Protective cover

Security screw

Adaptor retainer

Display screen/ Operation panel

Cover latch

Structure Overview - Back

ON/OFF button

Power supply plug

Foam

5. Installation Procedures

Unpacking and Packing List

• Carefully remove the UPHO mainframe, accessories and documentation

from the wooden shipping crate.

• Check that you have received all the item in the quantities on the list

below. If anything is missing, please contact your supplier. You will also find

detailed information on the contents of the package on the packing list in

the shipping crate.

UPHO mainframe

User and Cell Disruption Manuals

Fuses

Sample adaptor security screws

(NB. one screw may be in the mainframe)

Power cord

Quality control report

Warranty card

Quantity Name

• Inspect the unit for any damage that may have occurred during shipment.

If the equipment is damaged, report it to the carrier and contact your

supplier. Save all packaging material in the event a return is necessary.

• UPHO comes fully assembled, requiring a minimum set-up. Please install

the equipment on a clean, dry-stable surface far away from any compatible

electrical outlet.

• Allow some space around UPHO for proper ventilation. This unit is "FOR

INDOOR USE ONLY". Avoid operating in areas of excessive humidity or

extremes of temperature.

Removal of the Transport Security System

• A transport security system is located on the underside of UPHO:

comprised of 4 bolts (circled on picture below).

THIS SYSTEM MUST BE

REMOVED BEFORE OPERATING THE UPHO.

• Remove the 4 transport bolts from the UPHO by

TILTING THE UPHO

SLIGHTLY TO EXPOSE THE TRANSPORT BOLTS

(as seen in the picture) and

using a 13 mm x 16 mm metal wrench.

DO NOT TURN THE UPHO ON ITS

SIDE - THIS MAY LEAD TO INTERNAL DAMAGE.

• Please retain and carefully store the 4 transport bolts, as they will be

needed to reinstall if UPHO needs to be transported in the future.

• Operating UPHO with transport security system installed, or transporting

UPHO without the transport security device installed, can lead to damage

to the mechanical components of the instrument.

Sample Adapter Installation

• Make sure UPHO is connected to a suitable power outlet.

• Load the filled (sample, buffer and beads) and capped sample tubes into

the cavities of the sample adapter, so that they fit in the holes. It is preferred

to position the tubes symmetrically in the sample adaptor to reduce

vibration.

• When all sample tubes are loaded, place the sample cover on the sample

adaptor.

• Open the protective cover on the sample compartment cover by

releasing the cover latch, remove the security screw if present, and slide the

sample adapter (and cover) on to the adaptor retainer ensuring that the

sample adaptor fits over the rectangular metal locator on the adaptor

retainer.

• Thread the security screw on to the adaptor retainer tighten down until

the sample adaptor and cover are securely in position –

Security screw should by finger tight and not over tightened. Please note

that the sample adapter body may wear with prolonged use.

• Close protective cover on the sample compartment and secure with the

cover latch.

• Press PLAY button (on the touch-screen) to start running the selected

program.

• The program will not start if the protective cover is open or the cover

latch is not secured.

For hard samples, you can use the following adaptors and cans:

6. Operating Procedures

Suggestions and recommendations with the most common grinding

parameters (time, frequency, beads type and size) are listed on the “Cell

Disruption Guide”, provided with UPHO.

Automatic Program

Main Menu

When you power on UPHO (in the back of the device), the main menu

window will appear in the screen, together with a green light.

By touching the screen, you can execute several operations:

• Decelerate button: to decrease grinding speed

• Accelerate button: to increase grinding speed

• Play: to start the chosen program

• Stop: to stop the program

• Pause: to pause and restart a program

User-defined Program

UPHO allows the user to define a program. Please follow these steps:

1) On the main menu, touch the “SETUP” button. This window will appear;

2) On the “Choose” column, there are 10 boxes, each one of them

corresponding to a certain program defined by the user. When the user

touches a box, it will turn yellow.

3) After that, please touch the “Modify” button, at the bottom of the screen.

The “Modify” button will turn yellow as well, after you press it.

4) This will now allow the user to set the different parameters: Running

Time, Grinding Cycle State and Frequency.

Frequency

M - Minutes
S - Seconds

Number of grinding cycles during
running time. Recommended to always
set as “100”

5) To define a program name, touch the box on the “Group” column, in the

same line as the yellow “Choose” box.

6) After that, a pop-window will appear as follows:

7) Write the name of the program by touching the different keys. Click on

ENT button to set.

8) By touching the other boxes, the user can define other parameters such

as “Running Time - Minutes and Seconds”, “Grinding Cycle State” and

“Frequency”.

9) Once the user has defined the program, click the “Apply” button at the

bottom of the screen to save it.

10)Then, click on “Close”, the main display will appear. Click on PLAY button

to start the grinding process.

11)The user can always modify later its own programs, by choosing a

certain program on the Group column.

12)After a long period of time non-use time, the main menu will turn black.

7. Cleaning Maintenance

• Before cleaning your UPHO, be sure to switch the UPHO off at the main

power switch (back of the instrument) and disconnect your UPHO from the

power outlet.

• If solvents or saline, acidic, or alkaline solutions are spilt on UPHO wipe

them away immediately.

Run/S

Time

Frequency

• Do not use spray cleaning or disinfectant liquids onto surfaces of your

UPHO - use a soft cloth dampened with cleaner and / or disinfectant

• Do not autoclave any part of UPHO (except beads)

• The different adapters should be cleaned after use using mild detergents

or 70% ethanol, rinsed with distilled water, and then dried using paper

towels.

UPHO is supplied to you with a two-year Manufacturer’s warranty which

runs from the delivery date.

8. Troubleshooting

Common problem

No power

No display on the

screen

Touch screen shows
incomplete or ununsual
characteres

Possible cause

Action(s)

CPU needs to be reset

Turn off the UPHO using the Main Power Switch

located on the back of the instrument

Wait 30 seconds

Turn the UPHO back on

Check main voltage

Check that the voltage of the unit matches

that being delivered by the main power supply

Make sure the unit is plugged in properly

Check and replace fuses if blown

Change switch

On/Off switch broken

Faulty fuse

Others

Change fuse

Change switch

Contact supplier

Instrument does not run

Motor temperature above 65ºC

Wait for temperature to drop below 60ºC

Incorrect voltage selected

Power off the unit and select appropriate voltage.

If problem persists, contact supplier

Faulty Motor or Controller

Abnormal running noise

Security Screw not locked in place

Faulty bearing

Contact supplier

Secure Security Screw in place and lock down properly

Tube leakage

Speed is too high

High temperature or pressure

inside sample tubes

Reduce processing time or speed

Touch screen keys do not work

Plug connector loose

Faulty key

Contact supplier
Contact supplier

9. Warranty Statement

The Manufacturer’s warranty supplied with this UPHO does not cover wear

from normal use; it does not apply if the UPHO or parts of the UPHO have

been altered, repaired or replaced by anyone other than an employee of

Geneye or Geneye’s authorised Service Partners.

Warranty does not apply to any instrument which has been damaged

through accident, negligence, failure to follow operating instructions, or

from the use of electric currents or circuits other than those specified on

the plate affixed to the instrument, misuse, or abuse.

Geneye reserves the right to change, alter, modify, or improve any of its

instruments without any obligation whatever and to make corresponding

changes to any instrument previously sold or shipped.

10. Disposal

End of Life Disposal

Reuse, recycling and recovery. The presence of this label on a product

means that the product contains electrical or electronic materials and

therefore must not be disposed of as unsorted waste but instead is treated

separately. The presence of these materials may, if not disposed of properly,

have potential adverse effects on the environment and human health.

Within the European Union, operators are urged to recycle such products

when being replacedwith a newer version or when they have outlived their

useful lives.

11. Relevant Standards & Regulatory Requirements

11. CE CERTIFICATION

EC DECLARATION OF CONFORMITY

The UPHO flexible bead mill device, a product of Geneye, is designed for rapid and efficient
disruption of up to 64 samples simultaneously (Automated Samples Grinder)

The UPHO device is in conformity with the requirements of the following European
Directives (and their associated amendments):

The tests on the above designated product complies with the requirements of Council
Directive 2014/35/EU relating to Electrical Equipment designed for use within certain
voltage limits.

Warning: This is a class A product. In a domestic environment this product may cause radio

interference in which case the user may be required to take adequate me asures.

Signed:

Date : January 15th, 2018

Mr. B. Coissac

Title : Managing Director

For and on behalf of: Biowire Ltd Suite D 6/F, Ho Lee Commercial Building

38-44 D’Aguilar Street, Central,

Hong Kong

Product Description:

UPHO device

Product Part Number:

GY-U001

Machinery Directive

2006/42/EC

Electromagnetic Compatibility Directive (EMC)

2014/30/EU

Electrical Safety Low voltage Directive (LVD)

2014/35/EU

Harmonized standards

EN ISO 12100:2010,EN 61010-1:2010,
EN 61326-1:2013,EN 61000-3-2:2014,
EN 61000-3-3:2013

Restrictions on the use of Certain Hazardous
Substances (RoHS)

2011/65/EU

Registration, Evaluation, Authorization and
Restruction of chemicals (REACH)

1907/2006

Regulations

UPHO also complies with other applicable European Directives and

International Standards, particularly:

• The Waste Electrical and Electronic Equipment (WEEE) Directive and the

Reduction of Hazardous Substances (RoHS) Directive

• EN 61010-1: ‘Safety requirements for electrical equipment for

measurement, control and laboratory use. General requirements’

12. UPHO Accessories

Thank you for
choosing

UPHO

We are sure it will
become an integral
part of your laboratory!

Visit us on the Web at www.geneye.com

E-mail us at info@geneye.com

Because of technological developments related to the products, systems, and/or services
described herein, the data and procedures are subject to change without notice.
Please consult your Geneye representative or visit www.geneye.com to verify that this
information remains valid.
© 2017, Biowire Ltd.

IFUGYUPHOa January 2018

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