Germiphene E2O User Manual - User Manual

Full Text Searchable PDF User Manual

Why should I test my water when I am performing regular
cleaning/maintenance?

Testing is essential in assuring your waterline treatment is
doing its job. There are many factors that affect the efficacy of a
waterline cleaning product, including the water source itself or
even human error. Complete dental unit waterline care requires
not only waterline maintenance, but also shock treatments
and monitoring. Germiphene has two waterline testing kits,
E|Line

and E|One

. Contact your Germiphene representative

to find the best option for your office water.

How often should I monitor my dental unit waterlines?

There are no specific Canadian guidelines on the frequency
of dental unit waterline testing. Always check manufacturer’s
instructions first. Germiphene waterline testing kits, E|Line

and E|One

, recommend quarterly testing from at least one

line/source. Do not always test the same line/source. All lines/
sources should be tested at least once a year. In addition, the
cuspidor cupfill should be tested quarterly. During the warmest
quarter of the year, test monthly instead of quarterly. Ask your
Germiphene representative for scheduling tips.

Waterline

Germiphene Waterline Monitoring Kits

E|Line

Water Quality Check Kit

•

Monitor water in house
using test vials and a dry
block incubator

•

Results in 24 hours

•

Easy, colour coded results

•

2 Vials per test: (1) Total
Bacteria + Fungi AND (2) E. coli + faecal coliform

E|One

Water Quality Indicator

•

Fast results

•

Test E|One

vials in house

using:
(1) a 37°C dry block incubator –
30 minute results OR
(2) at room temperature –
1 hour results

•

One test vial for bacteria,
biofilm and fungi

•

Easy, colour coded results

Dental Waterline Maintenance

& Shock Treatment

First Time User Guide

For use in independent
dental water bottle systems.
Professional use only.
Use E2O

in conjunction

with a waterline test kit such

as E|Line

or E|One

Complete dental unit

waterline care requires:

shock treatments,
maintenance, and
monitoring.

E2O

can

be used as
both a daily

water maintenance

treatment, as well as a
shock treatment when

mixed at a higher
concentration.

E2O

Q&A

How safe is E2O

if ingested?

The proprietary ingredient in E2O

is HUWA-SAN®

Peroxide Technology, which is certified by NSF International
as a drinking water additive.
Hydrogen peroxide sold at your local pharmacy, and used
as a mouth rinse, contains 3% hydrogen peroxide which
is equivalent to 30 000 parts per million (ppm). The daily
treatment of E2O

is 1 pump (1.5mL) of product diluted

with 700mL of water, equivalent to 20 ppm. Even at the
concentrated shock level, the amount of peroxide is only 100
ppm. Remember that the shock treatment is not done during
patient procedures and the lines are purged afterwards.
A tooth whitening strip (directly placed in your mouth)
contains 500 times more hydrogen peroxide than E2O

(placed in your waterlines).

Why do I need to shock with E2O

if I am using the

product daily?

The 20 parts per million (ppm) hydrogen peroxide in your
E2O

daily maintenance treatment is enough to prevent

bacteria and biofilm from growing. However to remove
established biofilm, you need the 100 ppm hydrogen
peroxide E2O

shock treatment. This is why a shock

treatment is recommended before initial product use – to
clean out the waterlines; then shock should be performed
at least monthly as biofilm and bacteria can accumulate.
Total dental unit waterline care requires not only waterline
maintenance, but also shock treatments and monitoring.

Can E2O

be used as a shock treatment for other

waterline treatment products?

Yes, E2O

is an excellent way to shock your waterlines

regardless if you are using E2O

as your regular

maintenance product. E2O

shock treatment is compatible

with many other waterline treatments. After the E2O

shock

treatment, remember to purge your lines before continuing
with your regular waterline maintenance. Bleach based
products are incompatible with E2O

. If you are unsure

about the compatibility of E2O

with your maintenance

waterline product, contact Germiphene or your Germiphene
representative for more information.

1.800.265.9931

www.germiphene.com

Use

T-Cup to

prepare water

sample for

testing

Add required amount of E2O

to water bottle (dose

dependent on water bottle size). Proceed to fill water bottle
with potable water (tap water or distilled water) to regular fill.
Adding E2O

prior to filling bottle will reduce splatter and

ensure product is properly mixed.

E2O

Shock Treatment

E2O

Shock Treatment | Directions

Please note the shock treatment is measured per chair,

regardless of the size of your water bottle.
Shock treatment should be performed at the end of the day,
after the last patient AND on a day when someone will be
returning the following morning.

Empty any remaining solution from the water bottle.

Prepare shock treatment. Reference the appropriate
number of chairs you need to shock from the

Shock

Treatment Dosage Chart

and dilute only what you need,

there is no wasted product. Add required amount of
E2O

to empty water bottle, then dilute with potable

water (tap water or distilled water) appropriately.
Adding E2O

prior to filling bottle will reduce splatter

and ensure product is properly mixed.
As the shock treatment requires such a small amount of
product per chair, we recommend one of the following:

Mix enough for all chairs in one bottle, and then
divide appropriately between the bottles in each
operatory OR

ii.

Mix enough for all chairs in one bottle,
then travel to each operatory and purge the
appropriate amount of shock treatment through
the lines.

If the bottle is not completely full with shock treatment,
shake well to ensure the solution comes in contact with
all the interior surfaces of the bottle.

Reattach bottle.

Ensure no attachments are on the lines (handpieces,
air/water syringes, ultrasonic and/or piezo scalers).

E2O

Shock Treatment | Dosage Chart

E2O

Daily Maintenance Treatment

Directions

700mL water bottle: 1 pump E2O

2L water bottle: 3 pumps E2O

Number of Chairs

E2O

Water (mL)

1 pump

100–150

2 pumps

200–250

3 pumps

300–350

4 pumps

400–450

5 pumps

500–550

6 pumps

600–650

7 pumps

700–750

8 pumps

800–850

9 pumps

900–950

10 pumps

1000–1050

11 pumps

1100–1150

12 pumps

1200–1250

13 pumps

1300–1350

14 pumps

1400–1450

15 pumps

1500–1550

(100–150mL)

water

Per Chair

1 pump E2O

Place the lines into a sink and purge the lines for 2–3
minutes. This ensures that the shock treatment has
passed throughout the lines. Leave the shock treatment
in the lines overnight.

In the morning remove and empty the bottle containing
the shock treatment.

Fill the bottle with E2O

Daily Maintenance Treatment

Reattach bottle to unit.

As per current standards/guidelines, purge the lines into
a sink, with no attachments, for 2–3 minutes. Removing
the attachments is an important step, as you do not
want any debris from this treatment lodging in the
attachments.

Continue to use the E2O

daily maintenance treatment,

refilling as needed.

E2O

daily maintenance treatment/E2O

shock treatment

maintains efficacy for up to 2 weeks when diluted.

•

A higher concentration of E2O

than the daily

maintenance treatment.

•

When to perform a shock treatment:

Before initial use of E2O

to clean out your system.

Once a month; or as needed (if testing indicates

bacteria above acceptable levels).

When the unit has not been in use for 4 or more

days AND the lines are left without water and

waterline treatment.

Best Practices

All waterlines should be purged at the beginning of
each workday by flushing them thoroughly with water
for at least 2 to 3 minutes. Before purging is carried
out, handpieces, air/water syringes and ultrasonic
scalers should be removed from the waterlines.

Handpieces using water coolant should be run for 20
to 30 seconds after patient care in order to purge all
potentially contaminated air and water. The handpiece
should then be removed and, following cleaning
and disinfection of clinical contact surfaces, another
sterilized handpiece is to be attached for use with the
next patient.

Always follow manufacturer’s instructions for use.

Complete dental unit waterline care requires not only
continuous waterline maintenance, but also shock
treatments and monitoring.

When replacing the water bottle: Try to avoid touching
the pick up tube. Always wipe the pickup tube with a
clean cloth before placing it back into the bottle.

Staff compliance of waterline protocol.

MAINTAIN

Complete
Waterline

Care

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